Scientific Publications

119. Mickal Nawatha, Joseph Rogers, Steven M. Bonn, Ido Livneh, Betsegaw Lemma,Sachitanand M. Mali, Ganga B. Vamisetti, Hao Sun, Beatrice Bercovich, Yichao Huang, Aaron Ciechanover, David Fushman, Hiroaki Suga* and Ashraf Brik*, De novo macrocyclic peptides that specifically modulate Lys48-linked ubiquitin chains, Nature Chemistry, 2019, Accepted.

118. Roman Meledin and Ashraf Brik, Mirroring Life’s Building Blocks. Cell Chemical Biology, 2019, 26, 5, 645 - 651. PDF

117. Ashraf Brik, Ehud Keinan, Chemical Biology: Powerful Synergy Between Two Cultures, Israel Journal of Chemistry, 2019, 59, 3-6. PDF

116. Muhammad Jbara, Suman Maity, Ashraf Brik*, Examining Several Strategies for the Chemical Synthesis of Phosphorylated Histone H3 Reveals the Effectiveness of the Convergent Approach, European Journal of Organic Chemistry, 2019DOI: 10.1002/ejoc.201900257 (selected as a very important paper). PDF

115. Shay Laps#, Hao Sun#, Guy Kamnesky, Ashraf Brik* (# these authors contributed equally), Palladium Mediated Direct Disulfide Bond Formation in Proteins Containing S‐Acetamidomethyl‐Cysteine under Aqueous Conditions, Angewandte Chemie, 2019, International Edition, PDF

114. Michael Morgan, Muhammad Jbara, Ashraf  Brik, Cynthia Wolberger*, Semi-synthesis of ubiquitinated histone H2B with a native or non-hydrolyzable linkage, Methods in Enzymology, 2019 PDF

113. Hao Sun, Sachitanand Mali, Sumeet Singh, Roman Meledin, Ashraf Brik, Yong Tae Kwon, Yelena Kravtsova-Ivantsiv, Beatrice Bercovich, Aaron Ciechanover, Diverse Fate of Ubiquitin Chain Moieties: The Proximal is Degraded with the Target and the Distal Protects it from Removal and Recycles, PNAS, 2019, 116, 16, 7805-7812. PDF

112. Melesse Nune, Michael T Morgan, Zaily Connell, Laura McCullough, Muhammad Jbara, Hao Sun, Ashraf Brik, Tim Formosa*, Cynthia Wolberger*, FACT and Ubp10 collaborate to modulate H2B deubiquitination and nucleosome dynamics,   eLife, 2019, 8:e40988. PDF

111. Ganga B. Vamisetti, Roman Meledin, Pushparathinam Gopinath and Ashraf Brik*, A Halogen Substitution in Isoquinoline Scaffold Switches the selectivity of Inhibition between USP2 and USP7, ChemBioChem, 2019, 20, 282-286PDF

110. Muhammad Jbara#, Shay Laps#, Michael Morgan, Guy Kamnesky, Guy Mann, Cynthia Wolberger, Ashraf Brik* (# these authors contributed equally), Palladium Prompted On-Demand Cysteine Chemistry for the Synthesis of Challenging and Uniquely Modified Proteins, Nature Communications, 2018, 9:3154; Featured in Nature Communications Editors' Highlights. PDF

109. Muhammad Jbara, Emad Eid and Ashraf Brik*, Palladium mediated deallylation in fully aqueous conditions for native chemical ligation at aspartic and glutamic acid sites, Organic & Biomolecular Chemistry, 2018, 16, 4061-4064. PDF

108. Roman Meledin, Sachitanand M. Mali, Oded Kleifeld* and Ashraf Brik*, Activity-Based Probes Developed by Applying a Sequential Dehydroalanine Strategy on Expressed Proteins Reveal a Potential α-globin Modulating Deubiquitinase, Angewante Chemie, 2018International Edition, 57, 5645-5649. PDF

107. Muhammad Jbara, Hao Sun, Guy Kamnesky and Ashraf Brik*, Chemical Chromatin Ubiquitylation, Current Opinions in Chemical Biology, 2018, 45:18–26. PDF

106. Hao Sun, Roman Meledin, Sachitanand M. Mali and Ashraf Brik* , Total Chemical Synthesis of Ester-Linked Ubiquitinated Proteins Unravels their Behavior with Deubiquitinases, Chemical Science, 2018, 9, 1661-1665. PDF

105. Suman Kumar Maity, Muhammad Jbara, Guy Mann, Guy kamnesky and Ashraf Brik* ,Total Chemical Synthesis of Histones and their Analogues Assisted by Native Chemical Ligation and Palladium Complexes, Nature Protocols, 2017, 12(11):2293-2322. PDF

104. Pushparathinam Gopinath, Atif Mahammed, Tal Eilon-Shaffer, Mickal Nawatha, Shimrit Ohayon, Doron Shabat*, Zeev Gross* and Ashraf Brik*, Switching Futile para-Quinone to Efficient ROS Generator: Ubiquitin Specific Protease-2 Inhibition, Electrocatalysis and Quantification, ChemBioChem, 201718,1683 –1687, Highlighted in Cover Page. PDF

103. Muhammad Jbara, Noga Guttmann-Raviv, Suman Kumar Maity, Nabieh Ayoub* and Ashraf Brik*Total Chemical Synthesis of Methylated Analogues of Histone 3 Revealed KDM4D as a Potential Regulator of H3K79me3, Bioorganic and Medicinal Chemistry, 2017, 25(18):4966-4970. PDF

102. Muhammad Jbara, Suman kumar Maity, Ashraf Brik*, Palladium in the Chemical Synthesis and Modification of ProteinsAngewandte Chemie ,2017, International Edition, 56,10644 –10655. PDF

101. Somasekhar Bondalapati#, Emad Eid#, Sachitanand M. Mali, Cynthia Wolberger, Ashraf Brik*Total Chemical Synthesis of SUMO-2-Lys63-linked Diubiquitin Hybrid Chains Assisted by Removable Solubilizing Tags (# these authors contributed equally), Chemical Science, 2017, 8, 4027-4034. PDF

100. Sachitanand M. Mali, Sumeet K. Singh, Emad Eid, and Ashraf Brik*, Ubiquitin Signaling: Chemistry Comes to the RescueJournal of American Chemical Society2017, 139, 4971−4986. (Highlighted in Spotlights on Recent JACS Publications)PDF

99. Pushparathinam Gopinath and Ashraf Brik*, 2,2'-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride, e-EROS, 2016, DOI: 10.1002/047084289X.rn00922.pub2. PDF

98. Sumeet K. Singh#, Indrajit Sahu#, Sachitanand M. Mali, Hosahalli P. Hemantha, Oded Kleifeld, Michael H. Glickman*, and Ashraf Brik*; Synthetic uncleavable ubiquitinated proteins dissect proteasome deubiquitination and degradation and highlight distinctive fate of tetraubiquitin (# these authors contributed equally), Journal of American Chemical Society, 2016138 (49), pp 16004–16015. PDF

97. Pushparathinam Gopinath#, Atif Mahammed#, Shimrit Ohayon, Zeev Gross* and Ashraf Brik*; Understanding and predicting the potency of ROS-based enzyme inhibitors, exemplified by naphthoquinones and ubiquitin specific protease-2 (# these authors contributed equally), Chemical Science, 2016, 7, 7079-7086. PDF

96. Muhammad Jbara, Shay Laps, Suman Kumar Maity and Ashraf Brik*; Palladium Assisted Cleavage of Peptides and Proteins Containing a Backbone Thiazolidine Linkage, Chemistry - A European Journal, 2016, 22, 14851 - 14855 (hot paper). PDF

95. Suman Kumar Maity#, Guy Mann#, Muhammad Jbara, Shay Laps, Guy Kamnesky, and Ashraf Brik*; Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis (# these authors contributed equally), Organic Letters201618, 3026–3029, ACS Editor Choice. PDF

94. Roman Meledin, Sachitanand M. Mali, Sumeet Singh and Ashraf Brik*; Protein ubiquitination via Dehydroalanine: Development and Insights into the Diastereoselective 1,4-Addition Step, Organic & Biomolecular Chemistry, 201614, 4817 - 4823. PDF

93. Suman Kumar Maity#, Muhammad Jbara#, Shay Laps and Ashraf Brik*; Efficient Palladium Assisted One-pot and Rapid Deprotection of Cysteine(Acetamidomethyl) Following Native Chemical Ligation and/or Desulfurization to Expedite Chemical Protein Synthesis (# these authors contributed equally), Angewandte Chemie ,2016, 55, 8108 - 8112. PDF 

92. Muhammad Jbara#, Suman Kumar Maity#, Mallikanti Seenaiah and Ashraf Brik*, Palladium Mediated Rapid Deprotection of N-terminal Cysteine Under Native Chemical Ligation Conditions for the Efficient Preparation of Synthetically Challenging Proteins (# these authors contributed equally), Journal of American Chemical Society2016138 ,5069–5075. PDF

91. Pushparathinam Gopinath, Shimrit Ohayon, Mickal Nawatha and Ashraf Brik*, Chemical and Semisynthetic Approaches to Study and Target Deubiquitinases, Chemical Society Reviews, 2016, 45, 4171 - 4198PDF

90. Michael Morgan, Mahmood Haj-Yahya, Alison E. Ringel, Prasanthi Bandi, Ashraf Brik and Cynthia Wolberger*, Structural Basis for Histone H2B deubiquitination by SAGA DUB Module, Science, 2016, 351 725-728. PDF

89. Somasekhar Bondalapati#, Muhammad Jbara# and Ashraf Brik*; Expanding the Chemical Toolbox for the Synthesis of Large and Uniquely Modified Proteins (# these authors contributed equally), Nature Chemistry, 2016, 8407–418. PDF

88. Muhammad Jbara, Suman Kumar Maity, Michael Morgan, Cynthia Wolberger* and Ashraf Brik*: Total Chemical Synthesis of Phosphorylated Histone H2A at Tyr57 Reveals Insight into the Inhibition Mode of the SAGA Deubiquitinating Module, Angewandte Chemie ,2016, 554972-6. PDF

87. Suman Kumar Maity, Muhammad Jbara and Ashraf Brik*: Chemical and Semisynthesis of Modified Histones, Journal of Peptide Science, 2016, 22: 252–259 (Festschrift for the journal on occasion of the 70th birthday of Stephen BH Kent) DOI 10.1002/psc.2848. PDF

86. Roman Meledin, Sachitanand M. Mali and Ashraf Brik*; Pushing the Boundaries of Chemical Protein Synthesis: The Case of Ubiquitin Chains, Polyubiquitinated Peptides and Proteins; The Chemical Record, 2016, 16, 509–519. PDF.

85. Hosahalli P. Hemantha, Somasekhar Bondalapati, Sumeet Singh and Ashraf Brik*; Chemical synthesis of ubiquitin chains, Current Protocols in Chemical Biology, 2015, 7, 229-2448. PDF

84. Shimrit Ohayon, Maya Refua and Ashraf Brik*; Rapid optimization of labeled ubiquitinated peptides for monitoring deubiquitinases activities; Organic. Biomol. Chem., 2015,13, 8182-8186PDF

83. Suman Kumar Maity, Shimrit Ohayon, and Ashraf Brik*; Synthesis of N-Hydroxy Isopeptide Containing Proteins; Organic . Lett.201517 (14), pp 3438–3441. PDF

82. Mallikanti Seenaiah, Muhammad Jbara, Sachitanand M. Mali and Ashraf Brik*Convergent Versus Sequential Protein Synthesis: The Case of Ubiquitinated and Glycosylated H2B; Angewandte Chemie, Volume 54, Issue 42, pages 12374–12378October 12, 2015PDF.

81. Yelena Kravtsova-Ivantsiv, Inna Shomer, Victoria Cohen-Kaplan, Berend Snijder, Giulio Superti-Furga, Hedva Gonen, Thomas Sommer, Tamar Ziv, Arie Admon, Inna Naroditsky, Muhammad Jbara, Ashraf Brik, Eli Pikarsky, Yong Tae Kwon, Ilana Doweck, Aaron Ciechanover*; KPC1-Mediated Ubiquitination and Proteasomal Processing of NF-κB1 p105 to p50 Restricts Tumor Growth; Cell, 2015, 161, 2, p333–347 PDF.

80. Somasekhar Bondalapati, Wissam Mansour, Mark A. Nakasone, Suman Kumar Maity, Michael H. Glickman* and Ashraf Brik*Chemical Synthesis of Phosphorylated Ubiquitin and Diubiquitin Exposes Positional Sensitivities of E1-E2 Enzymes and Deubiquitinases; Chemistry - A European Journal, Volume 21, Issue 20, May 11, 2015, Pages 7360–7364PDF

79. Shimrit Ohayon, Maya Refua, Adi Hendler, Amir Aharoni, and Ashraf Brik, Harnessing the Oxidation Susceptibility of Deubiquitinases for Inhibition with Small Molecules; Angewandte Chemie Int. Ed., 2015, 54, 599-603. PDF

78. Ashraf Brik (Author Profile),  Angewandte Chemie Int. Ed., 2014, 53, 1738. PDF 

77. Muhammad Jbara, Mallikanti Seenaiah and Ashraf Brik*: Solid Phase Chemical Ligation Employing a Rink Amide Linker for the Synthesis of Histone H2B Protein, ChemComm, 2014, 50, 12534-7. PDF

76. Somasekhar Bondalapati, Emil Ruvinov, Olga Kryukov, Smadar Cohen* and Ashraf Brik*: Rapid End-Group Modification of Polysaccharides for Biomaterial Applications in Regenerative Medicine, Macromolecular Rapid Communications 2014, 35, 20, 1754-1762PDF

75. Daria Krutauz, Noa Reis, Mark A Nakasone, Peter Siman, Daoning Zhang, Donald S Kirkpatrick , Steven P Gygi, Ashraf Brik, David Fushman, Michael H Glickman: Extended ubiquitin species are protein-based DUB inhibitors, Nature Chemical Biology, 2014, 10(8), 664-U212. PDF

74. Lindsey Long, Joseph P Thelen, Melonnie Furgason, Mahmood Haj-Yahya, Ashraf Brik, Dongmei Cheng, Junmin Peng, and Tingting Yao*: The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination, J. Biol. Chem., 2014, 289(13), 8916-30. PDF

73. Hosahalli P. Hemantha, Sudhir N. Bavikar, Yifat Herman-Bachinsky, Najat Haj-Yahya, Somasekhar Bondalapati, Aaron Ciechanover, Ashraf Brik* Non-Enzymatic Polyubiquitination od Expressed proteins, J. Am. Chem. Soc. 2014, 136 (6),2665–2673. Highlighted 1) ACS Editor Choice; 2) Spotlights on Recent JACS Publications 3) ACS Chemical BiologyPDF

72. Christian F.W. Becker, Ashraf Brik, Phil Dawson and Christian P. R. Hackenberger; Chemical protein synthesis; J. Peptide Science 2014, 20, 2, 63. PDF

71. Najat-Haj Yahya, Roman Meledin, Hosahalli P. Hemantha, Somasekhar Bondalapati, Mallikanti Seenu, Ashraf Brik*: Dehydroalanine-Based Diubiquitin Activity Probes, Organic Letters, 2014, 16, 540–543. PDF

70. Mahmood Haj-Yahya, Bruno Fauvet, Yifat Herman-Bachinsky, Mirva Hejjaoui, Sudhir N. Bavikar, Subramanian Vedhanarayanan Karthikeyan, Aaron Ciechanover*, Hilal A. Lashuel*, Ashraf Brik*: Synthesis of tetra-ubiquitinated α-Synuclein reveals novel insights into the roles of ubiquitin chain in regulating pathophysiology, Proceeding National Academy of Science. USA, 2013, vol. 110 no.44, 17726–17731. Highlighted in 1) a commentary by Ivan Dikic et al in PNAS (Breaking the limits of artifical ubiquitination),PNAS, 2013, vol. 110 no.44, 17606–17607.2)News of the Week of C&EN. Issue: Vol. 91, #38, Sept. 23, 2013. PDF

69. Najat Haj-Yahya, Mahmood Haj-Yahya, Carlos A. Castañeda, Liat Spasser, Hosahalli P. Hemantha, Muhammad Jbara, Marlin Penner, Aaron Ciechanover,David Fushman and Ashraf Brik*: Modifying the Vicinity of the Isopeptide Bond to Reveal Differential Behavior of Ubiquitin Chains with Interacting Proteins: Organic Chemistry Applied to Synthetic Proteins, Angewandte Chemie Int. Ed. 2013, 52, 11149 –11153 PDF

68. Bruno Fauvet, Sara M. Butterfield, Jonas Fuks, Ashraf Brik, Hilal A. Lashuel*: One-pot total chemical synthesis of human alpha-synuclein, Chem.Commun.,2013,49,9254 PDF

67. Efrat Shema-Yaacoby, Miroslav Nikolov, Mahmood Haj-Yahya, Peter Siman, Eric Allemand, Yuki Yamaguchi, Christian Muchardt, Henning Urlaub, Ashraf Brik, Moshe Oren*, Wolfgang Fischle*: Systematic identification of proteins binding to chromatin-embedded ubiquitylated H2B reveals recruitment of the SWI/SNF complex to regulate transcription, Cell Reports, 2013, 4, 601-608 PDF

66. Peter Siman#, Subramanian Vedhanarayanan Karthikeyan#, Miroslav Nikolov, Wolfgang Fischle and Ashraf Brik*: Convergent Chemical Synthesis of Histone H2B Protein Enabled Site Specific Ubiquitination at Lys34; Angewandte Chemie Int. Ed. 2013,52, 8059-8063. Selected by the editors as a Hot Paper (# these authors contributed equally). PDF

65. Ashraf Brik*: Chemistry and biology of peptides, proteins and beyond; Bioorganic and Medicinal Chemistry, 2013, 21, 3398-3399; (Editorial, Special BMC issue in honor of the 2013 Tetrahedron Young Investigator Award winner Professor Ashraf Brik). PDF

64. Tal Moyal, Hosahalli Hemantha, Peter Siman, Maya Refua, Ashraf Brik*: Highly Efficient One-Pot Ligation and Desulfurization, Chemical Science, 2013, 4(6), 2496-2501. PDF

63. Hosahalli Hemantha and Ashraf Brik*: Non-Enzymatic Synthesis of Ubiquitin Chains: Where Chemistry Makes a Difference, Bioorganic and Medicinal Chemistry, 2013, 21, 3411-3420; (Special BMC issue in honor of the 2013 Tetrahedron Young Investigator Award winner Professor Ashraf Brik). PDF

62. Roman Meledin, Ashraf Brik,* Amir Aharoni,*: Dissecting the Roles of the N- and C-flanking Residues of Acetyl-lysine Substrate for SIRT1 activity, ChemBioChem, 2013, 14, 577–581. PDF

61. Mahmood Haj-Yahya, Najat Eltrteer, Shimirit Ohayon, Efrat Shema, Eran Kotler, Moshe Oren and Ashraf Brik*: N-methylation of Isopeptide Bond as a Strategy to Resist Deubiquitinases, Angewandte Chemie Int. Ed. 2012, 51(46), 11535-9. PDF

60. Tal Moyal, Sudhir Bavikar, Subramanian Vedhanarayanan Karthikeyan, Hosahalli Hemantha, Ashraf Brik*: Polymerization Behavior of a Bifunctional Ubiquitin Monomer as a Function of the Nucleophile Site and Folding Conditions, Journal of the American Chemical Society, 2012, 134(38),16085-16092. PDF

59. Liat Spasser, Ashraf Brik*: Chemistry and Biology of the Ubiquitin Signal (Invited Review), Angewandte Chemie Int. Ed. 2012, 51, 6840-6862. PDF

58. Nitzan Shabek, Yifat Herman-Bachinsky, Samuel Buchsbaum, Oded Lewinson, Mahmood Haj-Yahya, Mirva Hejjaoui, Hilal A. Lashuel,Thomas Sommer, Ashraf Brik, and Aaron Ciechanover*: The Size of the Proteasomal Substrate Determines whether its Degradation will be Mediated by Mono- or Polyubiquitylation, Molecular Cell, 2012, 48, 1-11. Highlighted in Nature Reviews Molecular Cell Biology 13, 602 (October 2012) doi:10.1038/nrm3445 PDF

57. Peter Siman, Subramanian Vedhanarayanan Karthikeyan and Ashraf Brik*: Native Chemical Ligation at Glutamine, Organic Letters, 2012, 14, 1520-1523. Highlighted in the Peptides virtual issue that compiles the most noteworthy recent research in the field that were published in Org Lett, JOC and JACS; September 7, 2012, Volume 1, Issue 2. PDF

56. Gilad Fuchs, Efrat Shema, Rita Vesterman, Eran Kotler, Zohar Lifshits, Sylvia Wilder, Lior Golomb, Ariel Pribluda, Feng Zhang, Mahmood Haj-Yahya, Ester Feldmesser, Ashraf Brik, Xiaochun Yu, Jacob Hanna, Daniel Aberdam, Eytan Domany and Moshe Oren*: RNF20 and USP44 regulate embryonic stem cell differentiation by modulating H2B monoubiquitylation, Molecular Cell, 2012, 46, 662-673. PDF

55. Peter Siman, Ashraf Brik*: Chemical and Semisynthesis of Posttranslationally Modified Proteins, Organic & Biomolecular Chemistry, 2012, 10, 5684-5697. PDF

54. Shimrit Ohayon, Liat Spasser, Amir Aharoni*, Ashraf Brik*: Targeting Deubiquitinases Enabled by Chemical Synthesis of Proteins, Journal of the American Chemical Society, 2012, 134, 3281-3289. PDF

53. Sudhir N. Bavikar, Liat Spasser, Mahmood Haj-Yahya, Subramanian Vedhanarayanan Karthikeyan, Tal Moyal, K. S. Ajish Kumar and Ashraf Brik*: Chemical Synthesis of Ubiquitinated Peptides with Varying Lengths and Types of Ubiquitin Chains to Explore the Activity of Deubiquitinases, Angewandte Chemie Int. Ed. 2012,51, 758–763. PDF

52. Ashraf Brik, Philip E. Dawson, Stephen BH Kent, Guest Editorial: Chemical Protein Synthesis, Israel Journal of Chemistry, 2011, 51, 852-853 PDF

51. Ashraf Brik*: Fighting Hepatitis C Virus with Peptide Nanotubes, Chemistry and Biology, 2011, 18, 1347-1348. (Invited Commentary) pdf

50. K. S. Ajish Kumar, Ashraf Brik*: δ-Mercaptolysine Assisted Ubiquitination, Israel Journal of Chemistry, 2011, 51, 900-907. (Special Issue on Protein Synthesis, Edited by Ashraf Brik, Philip E. Dawson, Stephen BH Kent). pdf

49. Carlos A. Castañeda, Liat Spasser, Sudhir N. Bavikar, Ashraf Brik*, David Fushman*: Segmental Isotopic Labelling of Ubiquitin Chains to Unravel Monomer-Specific Molecular Behavior, Angewandte Chemie Int. Ed. 2011, 50, 11210–11214. pdf

48. Natalie Zeytuni, Ertan Ozyamak, Kfir Ben-Harush, Geula Davidov, Maxim Levin, Yair Gat, Tal Moyal, Ashraf Brik, Arash Komeili, Raz Zarivach*: Self-Recognition Mechanism of MamA, a Magnetosome-Associated TPR-Containing Protein, Promotes Complex Assembly; Proceedings of the National Academy of Science, USA, 2011, 108, E480-E487 PDF

47. K. S. Ajish Kumar#, Sudhir N. Bavikar#, Liat Spasser, Shimrit Ohayon, Tal Moyal and Ashraf Brik*: Total Synthesis of a 304 residue, K48-Linked Tetraubiquitin, Angewandte Chemie, 2011,50, 6137-6141; (# these authors contributed equally). Selected by the editors as a Hot Paper. See highlight on this paper in News of the Week of C&EN, May 30, 2011, Volume 89, number 22, p. 7 PDF

46. Rinat Roytman, Lihi Adler-Abramovich, K. S. Ajish Kumar, Ting-Chun Kuan, Chun-Cheng Lin, Ehud Gazit*, Ashraf Brik*: Exploring the Self-Assembly of Glycopeptides Using Diphenylalanine Scaffold, Organic & Biomolecular Chemistry, 2011, 9, 5755-5761.

45. Peter Siman, Ofrah Blatt, Tal Moyal, Tsafi Danieli, Mario Lebendiker, Hilal A. Lashuel, Assaf Friedler*, Ashraf Brik*: Chemical Synthesis and Expression of the HIV-1 Rev Protein, ChemBioChem, 2011, 12, 1097-1104. PDF

44. K. S. Ajish Kumar, Liat Spasser, Shimrit Ohayon, Lesly Erlich, Ashraf Brik*:Expeditious Chemical Synthesis of Ubiquitinated Peptides Employing Orthogonal Protection, Native Chemical Ligation, Bioconjugate Chemistry (ACS publication), 2011, 22, 137-143. PDF

43. Mirva Hejjaoui#, Mahmood Haj-Yahya#, K. S. Ajish Kumar, Ashraf Brik*, Hilal A. Lashuel*: Towards elucidating the role of ubiquitination in the pathogenesis of Parkinson’s disease using semisynthetic ubiquitinated α-synuclein Angewandte Chemie Int. Ed. 2011, 50, 405. Selected by the editors as a Hot Paper (# these authors contributed equally).PDF

42. Liat Spasser, K. S. Ajish Kumar, Ashraf Brik*:Side-Chain Assisted Ligation: Scope and limitation, Journal of Peptide Science, 2011, 17, 252-255. PDF

41. K. S. Ajish Kumar#, Liat Spasser#, Lesly A. Erlich, Sudhir N. Bavikar, Ashraf Brik*: Total Chemical Synthesis of all di-Ubiqutin Chains, Angewandte Chemie Int. Ed. 2010,49, 9126-9131, (# these authors contributed equally). This communication has been selected as a frontispiece for Angewandte Chemie. See highlights about this paper: 1) C&EN, Vol 88 # 41, October 11, 2010; 2) Carpe Diubiquitin, by L. Martin & R. T. Raines, Angewandte Chemie Int. Ed. 2010,49, 9042-9044;3) C&EN, December 20, 2010, issue: Chemical Year in Review 2010. PDF

40. Ziv Harpaz, Peter Siman, K. S. Ajish Kumar, Ashraf Brik* : Protein Synthesis Assisted by Native Chemical Ligation at Leucine, ChemBioChem, 2010, 11, 1232-1235. PDF

39. K. S. Ajish Kumar, Ashraf Brik*: Accessing Posttranslationally Modified Proteins through Thiol Positioning, Journal of Peptide Science, 2010, 16, 524-529. PDF

38. Mahmood Haj-Yahya, K. S. Ajish Kumar, Lesly A Erlich, Ashraf Brik*: Protecting Group Variations of δ-Mercaptolysine Useful in Chemical Ubiquitylation, Biopolymers, 2010, 94, 504-510. PDF

37. Ashraf Brik*: New Strategies for Glycopeptides, Neoglycopeptides and Glycoproteins Synthesis, In Comprehensive Natural Products II Chemistry and Biology; Mander, L., Lui, H.-W., Eds.; Elsevier: Oxford, 2010; volume 6, pp.55–89.

36. Lesly A. Erlich, K. S. Ajish Kumar, Mahmood Haj-Yahya, Philip E. Dawson, Ashraf Brik*: N-Methylcysteine-Mediated Total Chemical Synthesis of Ubiquitin Thioester, Organic & Biomolecular Chemistry, 2010, 8, 2392-2396. PDF

35. K. S Ajish Kumar, Mahmood Haj-Yahya, Diana Olschewski, Hilal A. Lashuel, Ashraf Brik*: Highly Efficient and Chemoselective Peptide Ubiquitylation, Angewandte Chemie, 2009, 48, 8090-8094; See highlight on this paper in C&EN, November 16, 2009, Volume 87. PDF

34. K. S. Ajish Kumar, Ziv Harpaz, Mahmood Haj-Yahya, Ashraf Brik*: Side-Chain Assisted Ligation in Protein Synthesis, Bioorganic & Medicinal Chemistry Letters, 2009, 19, 3870–3874. PDF

33. Natalia Nepomniaschiy, Valerie Grimminger, Aviv Cohen, Saviana DiGiovanni, Hilal Lashuel*, Ashraf Brik*: Switch Peptide via Staudinger Reaction, Organic Letters, 2008, 11, 5243-5246. PDF

32. Ashraf Brik*: Metathesis in Peptides and Peptidomimetics, Advanced Synthesis and Catalysis, 2008, 350, 1661-1675. PDF

31. Marina-Yamit Lutsky, Natalia Nepomniaschiy, Ashraf Brik*: Peptide Ligation via Side-Chain Auxiliary, Chemical Communications, 2008, 10, 1229-1231. PDF

30. Michael J. Giffin, Holly Heaslet, Ashraf Brik, Ying-Chuan Lin, Gabrielle Cauvi1, Chi-Huey Wong, Duncan E. McRee, John H. Elder, C. David Stout, Bruce E. Torbett: AB2, an Azide-Alkyne Click Compound, is a Potent Protease Inhibitor of Multidrug-Resistant HIV-1, Journal of Medicinal Chemistry, 2008, 51, 6263-6267. PDF

29. Clay S. Bennett, Richard J. Payne, Simon Ficht, Stephen M. Dean, Ashraf Brik, Chi-Huey Wong: Sugar-Assisted Glycopeptide Ligation with Complex Oligosaccharides, Journal of The American Chemical Society, 2008, 130, 11945-11952.PDF

28. Chi-Huey Wong, Ashraf Brik, Yu-Ying Yang, Simon Ficht, Richard Payne: Method of preparing glycopeptides, PCT Int. Appl. (2007), CODEN: PIXXD2 WO 2007111952 A2 20071004 AN 2007:1116927.

27. Richard J. Payne, Simon Ficht, Sishi Tang, Ashraf Brik, Yu-Ying Yang, David A. Case, Chi-Huey Wong, “Extended Sugar-Assisted Glycopeptide Ligations: Development, Scope and Applications” Journal of The American Chemical Society, 2007, 129, 13527-13536. PDF

26. Yu-Ying, Yang, Simon Ficht, Ashraf Brik*, Chi-Huey Wong*: Sugar-Assisted Ligation in Glycoprotein Synthesis, Journal of the American Chemical Society, 2007, 129, 7690-7701. PDF

25. Ashraf Brik*, Chi-Huey Wong* “Sugar-Assisted Ligation for the Synthesis of Glycopeptides” Chemistry-A European Journal, 2007, 13, 5670-5675. PDF

24. Simon Ficht, Richard J. Payne, Ashraf Brik, Chi-Huey Wong: Second Generation Sugar-Assisted ligation: An effective New Method for the Synthesis of Cysteine Containing Glycopeptides, Angewandte Chemie International Edition, 2007, 46, 5975-5979. PDF

23. Ashraf Brik,* Simon Ficht, Yu-Ying Yang, Chi-Huey Wong*:Sugar Assisted ligation for the Synthesis of N-linked Glycopeptide with Broad Sequence Tolerance at the Ligation Junction, Journal of the American Chemical Society, 2006, 128, 15026-15033. See highlight about this paper in News & Views, Nature, 2007, 445, 31-33. PDF

22. Ashraf Brik, Simon Ficht, Chi-Huey Wong: Strategies for the Preparation of Homogenous Glycoproteins, Current Opinion in Chemical Biology, 2006, 10, 1-7. PDF

21. Ying-Chuan Lin, Ashraf Brik, Aymeric de Parseval, Karen Tam, Bruce Torbett, Chi-Huey Wong, John H. Elder: Altered Gag-Pol Polyprotein Cleavage Specificity of FIV/HIV Mutant Proteases as Demonstrated in Cell-Based Expression System, Journal of Virology, 2006, 80, 7832-7843. PDF

20. Ashraf Brik, Yu-Ying Yang, Simon Ficht, Chi-Huey Wong: Sugar Assisted Glycopeptide Ligation” Journal of The American Chemical Society, 2006, 128, 5626-5627. PDF

19. Ashraf Brik, Chung-Yi Wu and Chi-Huey Wong: Microtiter Plate Based Chemistry and In Situ Screening: A Useful Approach for Rapid Inhibitors Discovery, Organic & Biomolecular Chemistry, 2006, 4, 1446-1457. PDF

18. Fu-Sen Liang, Ashraf Brik, Ying-Chuan Lin, John H. Elder, and Chi-Huey Wong. “Epoxide Opening in Water for Rapid Inhibitor Discovery in Microtiter Plate and In situ screening” Bioorganic & Medicinal Chemistry, 2006, 14, 1058-1062 PDF

17. Chung-Yi Wu, Ashraf Brik, Sheng-Kai Wang, Yu-Hsien Chen, and Chi-Huey Wong: Tetrabutylammonium Fluoride-Mediated Rapid Alkylation Reaction in Microtiter Plates for Discovery of Enzyme Inhibitors In situ, ChemBiochem, 2005, 6, 2176-2180. PDF

16. Ashraf Brik, Jerry Alexandratos, Ying-Chuan Lin, John H. Elder, Arthur J. Olson, David S. Goodsell, Alexander Wlodawer and Chi-Huey Wong: 1,2,3 Triazole as a Peptide Bond Surrogate in the Rapid Synthesis of HIV Protease Inhibitors, ChemBiochem, 2005, 6, 1167-1169 (highlighted on the cover page). PDF

15. Ashraf Brik, Chung-Yi Wu, Michael D. Best and Chi-Huey Wong: Tetrabutylammonium Fluride-Assisted Rapid N9-Alkylation on Purine Ring: Application to Combinatorial reaction in Microtiter plates for the Discovery of Potent Sulfotransferase Inhibitors In situ, Bioorganic & Medicinal Chemistry, 2005, 13, 4622-4626. PDF

14. Ashraf Brik and Ehud Keinan, “Catalytic Antibodies in Natural Product Synthesis” Catalytic Antibodies, Ehud Keinan, ed. (WILEY-VCH), 2005, 132-152 PDF

13. Chung-Yi Wu, Jia-Tsrong Jan, Shiou-Hwa Ma, Chih-Jung Kuo, Hsueh-Fen Juan, Yih-Shyun E Cheng, Hsuan Cheng Huang, Douglass Wu, Ashraf Brik, Fu-Sen Liang, Rai-Shung Liu, Jim-Min Fang, Shui-Tein Chen Po-Huang Liang,Chi-Huey Wong: Small Molecules Targeting Severe Acute Respiratory Syndrome (SARS) Human Coronavirus, Proceedings of the National Academy of Science, USA, 2004, 101(27), 10012-10017. PDF

12. Norman Metanis, Ashraf Brik, Ehud Keinan, and Philip E. Dawson: Electrostatic interaction dominate the contribution of Arg39 in 44 Oxalocrotonate Tautomerase, Journal of the American Chemical Society, 2004, 126 (40), 12726-12727. PDF

11. Ting-Jen Chen, Ashraf Brik, Chi-Huey Wong and Chen-Chen Kan: A model System for High-Throughput Screening of Novel Human Immunodeficiency Virus (HIV) Protease Inhibitors in Escherichia Coli, Antimicrobial Agents and Chemotherapy, 2004, 48(7), 2437-2447. PDF

10. Michael D. Best, Ashraf Brik, Lac V-Lee, Ali Chapman, Wei-Chieh Cheng and Chi-Huey Wong: Rapid Discovery of Potent Sulfotransferase Inhibitors by Diversity-Oriented Reaction in Microplates Followed by in Situ Screening, ChemBioChem, 2004, 5, 811-819. PDF

9. Ashraf Brik, John Muldoon, Ying-Chuan Lin, John H. Elder, David S. Goodsell, Arthur J. Olson, Valery V. Fokin, K. Barry Sharpless and Chi-Huey Wong: A Rapid Diversity-Oriented Synthesis and In Situ Screening of HIV Protease Inhibitors, ChemBioChem, 2003, 4, 1246-1248. PDF

8. Chi Ching Mak, Ashraf Brik, Danica L. Lerner, John H. Elder, Garrett M. Morris, Arthur J. Olson,Chi-Huey Wong: Design and Synthesis of Broad-Based Mono- and Bi-cyclic Inhibitors of FIV and HIV Proteases, Bioorganic & Medicinal Chemistry, 2003, 11, 2025-2040. PDF

7. Ashraf Brik and Chi-Huey Wong: HIV Protease: Mechanism and Drug Discovery, Organic & Biomolecular Chemistry, 2003, 1, 5-14 (highlighted on the cover page),(Top 10 most downloaded OBC articles in 2006-2007 and 2009). PDF

6. Ashraf Brik, Ying-Chuan Lin, John Elder, Chi-Huey Wong, A Quick Diversity-Oriented Amide-Forming Reaction to Optimize p-subsite Residues of HIV Protease Inhibitors, Chemistry & Biology, 2002, 9, 891-896. PDF

5. Ashraf Brik, Lawrence J. D’Souza, Ehud Keinan, Flavio Grynszpan and Philip E. Dawson: Mutants of 4-Oxalocrotonate Tautomerase Catalyze the Decarboxylation of Oxaloacetate Through an Imine Mechanism, ChemBioChem, 2002, 3, 845-851, (highlighted on the cover page). PDF

4. Ashraf Brik, Philip E. Dawson and Ehud Keinan: The Product of the Natural Reaction Catalyzed by 4-oxalocrotonate Tautomerase Becomes an Affinity Label of its Mutant, Bioorganic & Medicinal Chemistry, 2002,10, 3891-3897. PDF

3. Ashraf Brik, Ehud Keinan, and Philip E. Dawson: Protein Synthesis by Solid-Phase Chemical Ligation Using a Safety Catch Linker, Journal of Organic Chemistry, 2000, 65, 3829-3835. PDF

2. Nizar Haddad, Michael Grishko, and Ashraf Brik: Studies Towards Total Synthesis of Borrelidin, Stereoselective Synthesis of the Polysubstituted Macrolidic Part, Tetrahedron Letters, 1997, 38, 6075-6078. PDF

1. Nizar Haddad, Ashraf Brik, and Michael Grishko: Studies Towards Total Synthesis of Borrelidin, Regioselective Methylation of Bis-epoxides and Structure Determination, Tetrahedron Letters 1997, 38, 6079-6082. PDF